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Actin Protein (MICAL-Oxidized): Rabbit Skeletal Muscle (Rabbit Muscle, > 95% Pur
| 貨號(hào) | MXA95 | 售價(jià)（元） | 4936 |
| 規(guī)格 | 2 x 250 μg | CAS號(hào) |
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MICAL interacts with F-actin and uses NADPH as a cofactor to oxidize actin at Met44 and Met47 (b-actin nomenclature). Functionally, oxidation of Met44 has a profound effect on actin polymerization because the residue resides in the D-loop of subdomain 2 of the protein, which is critical for actin subunit contacts; thus, upon oxidation, Met44 becomes negatively charged and interferes with actin monomer-monomer interaction and promotes F-actin severing and depolymerization. Regulation of actin oxidation at Met44/Met47 has been shown to destabilize F-actin in vivo and to play a key role in a growing number of cellular processes. As part of the MOXtrue? product line, rabbit skeletal muscle actin protein (MICAL-oxidized) (MXA95) has been enzymatically oxidized at methionines 44 and 47 with the MICAL flavoprotein monoxygenase protein. Purified MICAL-oxidized actin has reduced susceptibility to subtilisin A cleavage at M47/G48 by > 90%, and has also been validated for downstream applications such as sedimentation assays.
To learn more about using MICAL-oxidized Actin as a research tool see our datasheet
To learn more about the MICAL/MsrB/Actin physiological redox system see our Newsletter
Each lot of purified protein is quality controlled to provide high batch to batch consistency, see COA documents.